Original Articles Vol. 3 No. 01 (2026): Bioscience Insights Published 2026-06-30

ISOLATION AND PHENOTYPIC CHARACTERIZATION OF MULTI-DRUG-RESISTANT PSEUDOMONAS AERUGINOSA FROM HEALTHCARE SETTING

Authors

  • Ambreen Aslam Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara, 56130 Pakistan Pakistan Author
  • Sabeen Sabri Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara, 56130 Pakistan Pakistan Author
  • Arfat Yousaf Shaheen Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara, 56130 Pakistan Pakistan Author
  • Furqan Ali Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara, 56130 Pakistan Pakistan Author
  • Ariha Afsheen Department of Microbiology and Molecular Genetics, Faculty of Life Sciences, University of Okara, Okara, 56130 Pakistan Pakistan Author
  • Muhammad Shahid khan Department of Physics, University of Okara, Okara, 56130 Pakistan Pakistan Author
  • Muhammad Saleem Khan Department of Zoology, Faculty of Life Sciences, University of Okara, Okara, 56130 Pakistan Pakistan Author
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Abstract

Multidrug-resistant (MDR) Pseudomonas aeruginosa is an important cause of healthcare-associated infections, particularly among critically ill and immunocompromised patients. The development of resistance to multiple antimicrobial classes creates a serious challenge for effective infection control. A total of 50 clinical and hospital environmental samples were obtained from the Bahria International Hospital, Lahore. The isolates were identified as P. aeruginosa using conventional microbiological procedures, including growth on selective culture media, Gram staining, and biochemical tests. Antibiotic susceptibility was evaluated by the Kirby-Bauer disc diffusion method using commonly prescribed antibiotics, and isolates resistant to three or more classes of antimicrobial agents were classified as multidrug resistant according to the established guidelines. P. aeruginosa was detected in 11 (22%) out of 50 samples analyzed. Antibiotic susceptibility testing showed that isolates were resistant to several antibiotics. Resistance was 100% to Cefepime, Imipenem, and Levofloxacin; 90.9% to Polymyxin B and Colistin; 72.7% to Meropenem and Tobramycin; 63.6% to Gentamicin; 36.4% to Piperacillin and 27.3% to Ciprofloxacin and Aztreonam. These findings showed the prevalence of MDR P. aeruginosa in both clinical and hospital environmental samples. The high level of antimicrobial resistance observed among the isolates highlights an increasing threat of MDR pathogens in healthcare settings. Regular microbiological surveillance, antimicrobial susceptibility testing, strict infection control practices, and antimicrobial stewardship programs are important to reduce the spread of MDR P. aeruginosa, improve patient care, and support appropriate empirical antibiotic therapy.

Keywords

  • MDR P. aeruginosa, AMR, Intensive Care Units (ICU), Ventilator-Associated Pneumonia (VAP), Beta-lactams (β-lactams)

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